QUANTITATIVE HISTOCHEMICAL-STUDY OF THE OPTIMAL INCUBATION CONDITIONS FOR CERTAIN OXIDATIVE-ENZYMES IN HAMSTER-CHEEK POUCH EPITHELIUM

被引:25
作者
EVANS, AW [1 ]
BUTCHER, RG [1 ]
机构
[1] LONDON HOSP,COLL MED,DEPT ORAL PATHOL,LONDON E1 2AD,ENGLAND
基金
英国医学研究理事会;
关键词
D O I
10.1016/0003-9969(79)90171-7
中图分类号
R78 [口腔科学];
学科分类号
1003 ;
摘要
The assay conditions of three oxidative enzymes, lactate, succinate and glucose-6-phosphate dehydrogenases, were examined histochemically and their activities quantified by scanning and integrating microdensitometry. Optimal concentrations of substrate, co-enzyme, tetrazolium salt and of the intermediate acceptor, phenazine methosulphate, were determined for each enzyme. When studied at pH 7.5, non-specific reactions in the keratinized layer were absent and control activities in the absence of either substrate or co-enzyme were low in the other epithelial layers. © 1979.
引用
收藏
页码:27 / 33
页数:7
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