PROTEASE-CATALYZED PEPTIDE-SYNTHESIS IN AQUEOUS-ORGANIC 2-PHASE SYSTEMS - REACTANT PRECIPITATION AND INTERFACIAL INACTIVATION

被引：21

作者：

CASSELLS, JM ^{[1
]}

HALLING, PJ ^{[1
]}

机构：

[1] UNIV STRATHCLYDE,DEPT BIOSCI & BIOTECHNOL,GLASGOW G1 1XW,SCOTLAND

来源：

ENZYME AND MICROBIAL TECHNOLOGY | 1990年 / 12卷 / 10期

关键词：

amino acid complexes; biphasic systems; pepsin; reaction rate; Thermolysin;

D O I：

10.1016/0141-0229(90)90147-I

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Peptides can be efficiently synthesized by the proteases thermolysin and persin acting in aqueousorganic two-phase systems similar to those reported previously. However, a number of problems can prevent successful reactions in these systems, with these and other proteases: (1) Complexes between amino acid reactants that are sparingly soluble in both phases may be precipitated, though their solubility behavior is complex; (2) the enzyme may be subject to rapid inactivation by contact with the phase interface, and the rate of this is unusually dependent on the nature of the solvent; and (3) the reaction rate is usually slow. © 1990.

引用

页码：755 / 759

页数：5

共 28 条

[11] JAMES LK, 1966, ADV ENZYMOL RAMB, V28, P1
[12] OPTIMIZATION OF ENZYME CATALYZED PEPTIDE-SYNTHESIS IN A WATER - WATER-IMMISCIBLE ORGANIC-SOLVENT BIPHASIC SYSTEM
KHMELNITSKI, YL
DIEN, FK
SEMENOV, AN
MARTINEK, K
VERUOVIC, B
KUBANEK, V
[J]. TETRAHEDRON, 1984, 40 (21) : 4425 - 4432
[13] ENZYME-CATALYZED PEPTIDE-SYNTHESIS IN BIPHASIC AQUEOUS-ORGANIC SYSTEMS
KUHL, P
KONNECKE, A
DORING, G
DAUMER, H
JAKUBKE, HD
[J]. TETRAHEDRON LETTERS, 1980, 21 (10) : 893 - 896
[14] RULES FOR OPTIMIZATION OF BIOCATALYSIS IN ORGANIC-SOLVENTS
LAANE, C
BOEREN, S
VOS, K
VEEGER, C
[J]. BIOTECHNOLOGY AND BIOENGINEERING, 1987, 30 (01) : 81 - 87
[15] MATSUBARA H, 1970, METHOD ENZYMOL, V19, P642
[16] USING PROTEASES IN PEPTIDE-SYNTHESIS
MORIHARA, K
[J]. TRENDS IN BIOTECHNOLOGY, 1987, 5 (06) : 164 - 170
[17] THE USE OF HYDROLASES IN ORGANIC-CHEMISTRY - SYNTHESIS OF AMIDE AND ESTER BONDS CATALYZED BY INDUSTRIAL PAPAIN
MORINIERE, JL
DANREE, B
GUY, A
[J]. EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY, 1987, 22 (04) : 347 - 357
[18] KINETICS AND EQUILIBRIUM OF ENZYMATIC-SYNTHESIS OF PEPTIDES IN AQUEOUS ORGANIC BIPHASIC SYSTEMS - THERMOLYSIN-CATALYZED SYNTHESIS OF N-(BENZYLOXYCARBONYL)-L-ASPARTYL-L-PHENYLALANINE METHYL-ESTER
NAKANISHI, K
KIMURA, Y
MATSUNO, R
[J]. EUROPEAN JOURNAL OF BIOCHEMISTRY, 1986, 161 (03): : 541 - 549
[19] CONTINUOUS SYNTHESIS OF N-(BENZYLOXYCARBONYL)-L-ASPARTYL-L-PHENYLALANINE METHYL-ESTER WITH IMMOBILIZED THERMOLYSIN IN AN ORGANIC-SOLVENT
NAKANISHI, K
KAMIKUBO, T
MATSUNO, R
[J]. BIO-TECHNOLOGY, 1985, 3 (05): : 459 - 464
[20] KINETICS OF ENZYMATIC-SYNTHESIS OF PEPTIDES IN AQUEOUS ORGANIC BIPHASIC SYSTEMS - THERMOLYSIN-CATALYZED SYNTHESIS OF N-(BENZYLOXYCARBONYL)-L-PHENYLALANYL-L-PHENYLALANINE METHYL-ESTER
NAKANISHI, K
MATSUNO, R
[J]. EUROPEAN JOURNAL OF BIOCHEMISTRY, 1986, 161 (03): : 533 - 540

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