CHEMOENZYMIC PREPARATION OF A GLYCOCONJUGATE POLYMER HAVING A SIALYLOLIGOSACCHARIDE - NEU5AC-ALPHA(2-]3)GAL-BETA(1-]4)GLCNAC

被引：44

作者：

NISHIMURA, SI

LEE, KB

MATSUOKA, K

LEE, YC

机构：

[1] JOHNS HOPKINS UNIV,DEPT BIOL,BALTIMORE,MD 21218

[2] HOKKAIDO UNIV,GRAD SCH SCI,DIV BIOL SCI,SAPPORO,HOKKAIDO 060,JAPAN

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1994年 / 199卷 / 01期

关键词：

D O I：

10.1006/bbrc.1994.1221

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Water-soluble polyacrylamide having 3'-sialyl N-acetyl-lactosamine [Neu5Ac alpha(2->3)Gal beta(1->4)GlcNAc] was enzymatically prepared by stepwise sugar-elongation on a water-soluble GlcNAc-bearing polyacrylamide. It was demonstrated that the flexible GlcNAc branches of the polymer chains allow quantitative galactosylation with bovine galactosyl transferase and partial sialylation by Trypanosoma cruzi trans-sialidase. Unsialylated N-acetyl-lactosamine side chains can be removed with beta-D-galactosidase and N-acetyl-beta-D-glucosaminidase. (C) 1994 Academic Press, Inc.

引用

页码：249 / 254

页数：6

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