COMPLETE NUCLEOTIDE-SEQUENCES OF 7 EUBACTERIAL GENES-CODING FOR THE ELONGATION FACTOR-TU - FUNCTIONAL, STRUCTURAL AND PHYLOGENETIC EVALUATIONS

被引：59

作者：

LUDWIG, W ^{[1
]}

WEIZENEGGER, M ^{[1
]}

BETZL, D ^{[1
]}

LEIDEL, E ^{[1
]}

LENZ, T ^{[1
]}

LUDVIGSEN, A ^{[1
]}

MOLLENHOFF, D ^{[1
]}

WENZIG, P ^{[1
]}

SCHLEIFER, KH ^{[1
]}

机构：

[1] TECH UNIV MUNICH,LEHRSTUHL MIKROBIOL,ARCISSTR 21,W-8000 MUNICH 2,GERMANY

来源：

ARCHIVES OF MICROBIOLOGY | 1990年 / 153卷 / 03期

关键词：

Elongation factor Tu; Eubacteria; Functional and structural elements of EF-Tu; Phylogenetic relationship; Tuf gene;

D O I：

10.1007/BF00249075

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The nucleotide sequences of cloned genes coding for the elongation factor Tu of seven eubacteria have been determined. These genes were fiom Anacystis nidulans, Bacillus subtilis, Bacteroides fragilis, "Deinonema" spec., Pseudomonas cepacia, Shewanella putrefaciens and Streptococcus oralis. The primary structures of the genes were compared to the available sequences of prokaryotic elongation factors Tu and eukaryotic elongation factors 1 alpha. A conservation profile was determined for homologous amino acid residues. Sites of known or putative functions are usually located at highly conserved positions or within highly conserved sequence stretches. The aligned 24 amino acid sequences were used as basis for a phylogenetic analysis. The phylogenetic tree corroborates the kingdom as well as phylum concept deduced from 16S rRNA data. © 1990 Springer-Verlag.

引用

页码：241 / 247

页数：7