STRUCTURAL STUDY OF A CELL-WALL MANNAN OF SACCHAROMYCES-KLUYVERI IFO-1685 STRAIN - PRESENCE OF A BRANCHED SIDE-CHAIN AND BETA-1,2-LINKAGE

被引：35

作者：

SHIBATA, N ^{[1
]}

KOJIMA, C ^{[1
]}

SATOH, Y ^{[1
]}

SATOH, R ^{[1
]}

SUZUKI, A ^{[1
]}

KOBAYASHI, H ^{[1
]}

SUZUKI, S ^{[1
]}

机构：

[1] TOHOKU COLL PHARMACEUT SCI, DEPT HYG CHEM 2, 4-4-1 KOMATSUSHIMA, AOBA KU, SENDAI, MIYAGI 983, JAPAN

来源：

EUROPEAN JOURNAL OF BIOCHEMISTRY | 1993年 / 217卷 / 01期

关键词：

D O I：

10.1111/j.1432-1033.1993.tb18211.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Acetolysis of the cell-wall mannan of Saccharomyces kluyveri under mild conditions, gave fragments with 1-6 mannose residues. The structures of mannopentaose and mannohexaose were determined to be [GRAPHICS] respectively, by two-dimensional homonuclear Hartmann-Hahn spectroscopy and a sequential NMR assignment method that combines H-1-C-13 correlated spectroscopy, relayed coherence transfer spectroscopy, H-1-detected heteronuclear multiple-bond connectivity and methylation analysis. The H1 proton chemical shift of a neighboring a-1,2-linked mannose unit of the 3-O-substituted structure was shifted upfield by the addition of a mannose unit to the adjacent 3-O-substituted unit by an alpha-1,6 linkage. The characteristic HI-H2-correlated cross-peak of the alpha-1,3-linked mannose unit substituted by a beta-1,2 linkage, beta1-->2Manalpha1-->3, in the mannan of S. kluyveri, as also found by two-dimensional homonuclear Hartmann-Hahn spectroscopy in the mannan of Candida guilliermondii, a pathogenic yeast in man.

引用

页码：1 / 12

页数：12

共 39 条

[1]

AMES BN, 1960, J BIOL CHEM, V235, P769

[2]

BALLOU L, 1989, J BIOL CHEM, V264, P11857

[3] MLEV-17-BASED TWO-DIMENSIONAL HOMONUCLEAR MAGNETIZATION TRANSFER SPECTROSCOPY [J].