STUDIES ON SUGAR-ISOMERIZING ENZYME - PURIFICATION, CRYSTALLIZATION AND SOME PROPERTIES OF GLUCOSE ISOMERASE FROM STREPTOMYCES SP

Glucose isomerase was purified by means of acetone fractionation, DEAE-cellulose column chromatography, DEAE-Sephadex column chromatography and crystallization. The purified enzyme appeared to be homogeneous on ultracentrifugation and electrophoresis. The sedimentation coefficient, s20,w, the diffusion coefficient, D20,w, and partial specific volume of the enzyme were 8.0S, 4 × 10‒7cm2/sec and 0.69 ml/g, respectively. The molecular weight of the enzyme was estimated to be 157,000 from the sedimentation and diffusion measurements. The crystalline glucose isomerase contained cobalt and magnesium ions. The properties of the enzyme were also studied. © 1969 Taylor and Francis Group LLC.