INVOLVEMENT OF A NON-BAY-REGION DIOL-EPOXIDE IN THE FORMATION OF BENZ(A)ANTHRACENE-DNA ADDUCTS IN A RAT-LIVER MICROSOMAL SYSTEM

被引：23

作者：

MACNICOLL, AD

COOPER, CS

RIBEIRO, O

GERVASI, PG

HEWER, A

WALSH, C

GROVER, PL

SIMS, P

机构：

[1] Chester Beatty Research Institute, Institute of Cancer Research: Royal Cancer Hospital, London, SW3 6JB England, Fulham Road

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1979年 / 91卷 / 02期

关键词：

D O I：

10.1016/0006-291X(79)91548-1

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The metabolic activation of benz(a)anthracene was investigated by incubating [3H]-benz(a)anthracene with DNA, a NADPH-generating system and rat-liver microsomes. When hydrolysates of the DNA were chromatographed on Sephadex LH20 columns, three hydrocarbon-nucleoside adduct peaks were resolved and these were further examined using HPLC. One adduct probably results from the reaction of the non-bay-region diol-epoxide r-8,t-9-dihydroxy-t-10,11-oxy-8,9,10,11-tetrahydrobenz(a)anthracene (anti-BA-8,9-diol 10,11-oxide) with DNA. The other two adducts did not co-chromatograph with adducts formed from any of the four possible isomeric diolepoxides that can be formed in the 8,9,10,11-ring of benz(a)anthracene. © 1979.

引用

页码：490 / 497

页数：8

共 16 条

[11] METABOLIC ACTIVATION OF BENZO(A)PYRENE PROCEEDS BY A DIOL-EPOXIDE
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[14] VIGNY P, UNPUBLISHED
[15] TUMORIGENICITY OF 5 DIHYDRODIOLS OF BENZ[A]ANTHRACENE ON MOUSE SKIN - EXCEPTIONAL ACTIVITY OF BENZ[A]ANTHRACENE 3,4-DIHYDRODIOL
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[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1977, 74 (08) : 3176 - 3179
[16] HIGH MUTAGENICITY OF METABOLICALLY ACTIVATED CHRYSENE 1,2 DIHYDRODIOL - EVIDENCE FOR BAY REGION ACTIVATION OF CHRYSENE
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