SOLUTION CONFORMATION OF LEIUROTOXIN-I (SCYLLATOXIN) BY H-1 NUCLEAR MAGNETIC-RESONANCE - RESONANCE ASSIGNMENT AND SECONDARY STRUCTURE

被引：56

作者：

MARTINS, JC

ZHANG, WG

TARTAR, A

LAZDUNSKI, M

BORREMANS, FAM

机构：

[1] STATE UNIV GHENT,ORGAN CHEM LAB,KRIJGSLAAN 281,S4BIS,B-9000 GHENT,BELGIUM

[2] INST PASTEUR,SERV CHIM BIOMOLEC,F-59019 LILLE,FRANCE

[3] INST PHARMACOL MOLEC & CELLULAIRE,F-06560 VALBONNE,FRANCE

来源：

FEBS LETTERS | 1990年 / 260卷 / 02期

关键词：

Apamin; NMR; 2-dimensional; Scorpion toxin; Structure; secondary;

D O I：

10.1016/0014-5793(90)80115-Y

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A proton NMR study at 500 MHz of leiurotoxin I in water is presented. Nearly complete sequence-specific assignments of the individual backbone and side-chain proton resonances were achieved using through-bond and through-space connectivities obtained from standard two-dimensional NMR techniques. The secondary structure of this toxin is inferred from a combination of short-range nuclear Overhauser enhancements, scalar couplings and proton/deuteron exchange rates. Three disulflde bridges locate the N-terminal part (that is α-helical from residue 6 to 16) on one side of a C-terminal two stranded antiparallel β sheet (from Leu18 to Val29). The latter features a tight turn at Gly23-Asp24. © 1990.

引用

页码：249 / 253

页数：5