BINDING OF CALCIUM TO FIBRINOGEN - INFLUENCE ON THE CLOTTING PROCESS

被引：27

作者：

MARGUERIE, G ^{[1
]}

BENABID, Y ^{[1
]}

SUSCILLON, M ^{[1
]}

机构：

[1] CEN, INST RECH FONDAMENTALE, GEOPHYS LAB, F-38041 GRENOBLE, FRANCE

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1979年 / 579卷 / 01期

关键词：

Ca[!sup]2+[!/sup; Fibrin monomer; Fibrinogen; Fibrinogen-fibrin complex;

D O I：

10.1016/0005-2795(79)90093-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The influence of Ca2+ on the basic reaction between thrombin and fibrinogen was investigated. The results demonstrate that: 1. (a)|A Ca2+-dependent dimeric intermediate is formed during the early step of the clotting process. This dimeric intermediate is shown to be formed by the association of an intact fibrinogen molecule and a fibrin monomer devoid in only the peptide A, 2. (b)|Ca2+ enhances the proteolytic step as illustrated by the measure of the kinetics of H+ release at pH 8.6. On the basis of these observations it is proposed that Ca2+ catalyses the proteolysis of fibrinogen by thrombin through the formation of a Ca2+-dependent dimer. © 1979.

引用

页码：134 / 141

页数：8