HIGH-PERFORMANCE ZONE ELECTROPHORESIS

被引：731

作者：

MIKKERS, FEP

EVERAERTS, FM

VERHEGGEN, TPEM

机构：

[1] Department of Instrumental Analysis, Eindhoven University of Technology, Eindhoven

来源：

JOURNAL OF CHROMATOGRAPHY | 1979年 / 169卷 / FEB期

关键词：

D O I：

10.1016/0021-9673(75)85029-1

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

An experimental approach to high-performance zone electrophoresis is given. It is shown that dispersion can be well controlled by the use of narrow-bore tubes made of chemically and electrically inert materials. The asymmetric concentration distributions that are frequently obtained in free zone electrophoresis are the result of migrational dispersion. This asymmetry only can be suppressed by the application of very small amounts of sample. High-performance separations with UV and conductimetric detection are shown. The time of analysis can be reduced to a few minutes by selecting the appropriate operational conditions. Plate heights smaller than 10 μm can easily be obtained. © 1979.

引用

页码：11 / 20

页数：10

共 20 条

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[5] EVERAERTS FM, 1968, THESIS EINDHOVEN U T
[6] GENERATION OF VARIANCE, THEORETICAL PLATES, RESOLUTION, AND PEAK CAPACITY IN ELECTROPHORESIS AND SEDIMENTATION
GIDDINGS, JC
[J]. SEPARATION SCIENCE, 1969, 4 (03): : 181 - &
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[9] HJERTEN S, 1967, THESIS U UPPSALA
[10] MULTIPHASIC ZONE ELECTROPHORESIS .1. STEADY-STATE MOVING-BOUNDARY SYSTEMS FORMED BY DIFFERENT ELECTROLYTE COMBINATIONS
JOVIN, TM
[J]. BIOCHEMISTRY, 1973, 12 (05) : 871 - 878

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