ANALYSIS OF TAXOL AND RELATED TAXANES FROM TAXUS-CANADENSIS USING LIQUID-CHROMATOGRAPHY COMBINED WITH MASS-SPECTROMETRY OR TANDEM MASS-SPECTROMETRY

被引:25
作者
BLAY, PKS
THIBAULT, P
THIBERGE, N
KIECKEN, B
LEBRUN, A
MERCURE, C
机构
[1] NATL RES COUNCIL CANADA,ATLANTIC REG LAB,INST MARINE BIOSCI,1411 OXFORD ST,HALIFAX B3H 3Z1,NS,CANADA
[2] BIOLYSE PHARMACOPEA INT CORP,PT DANIEL G0C 2N0,PQ,CANADA
关键词
D O I
10.1002/rcm.1290070715
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
A liquid chromatography/mass spectrometry (LC/MS) method using nebulizer-assisted electrospray (ionspray) is described for the analysis of taxol and related taxane analogs in plant extracts from Taxus canadensis. The method used a reverse-phase liquid chromatography gradient elution which was entirely compatible with the operation of ionspray ionization. Abundant protonated molecules with limited fragmentation were obtained using this ionization technique, and provided adequate sensitivity for monitoring taxol in crude resin extracts. The LC/MS method was evaluated for the analysis of taxol present at levels of 0.2% in resin extracts. Using selected-ion monitoring, the method provided detection limits of 50 ng/mL. In addition, other taxane analogs were identified in crude resin extracts using liquid chromatography combined with tandem mass spectrometry. Parent-ion scans for a fragment ion characteristic of the taxane nucleus provided the necessary selectivity and sensitivity for the identification of potentially active diterpenes in complex extracts.
引用
收藏
页码:626 / 634
页数:9
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