STAGE TRANSITIONS IN LYMPHOCYTE-B DIFFERENTIATION CORRELATED WITH LIMITED VARIATIONS IN NUCLEAR PROTEINS

被引：17

作者：

RABILLOUD, T ^{[1
]}

PENNETIER, JL ^{[1
]}

HIBNER, U ^{[1
]}

VINCENS, P ^{[1
]}

TARROUX, P ^{[1
]}

ROUGEON, F ^{[1
]}

机构：

[1] ECOLE NORM SUPER, DEPT BIOL, UNITE PHYSIOL & BIOCHIM DEV 686, F-75231 PARIS 05, FRANCE

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1991年 / 88卷 / 05期

关键词：

2-DIMENSIONAL ELECTROPHORESIS; GENETIC EXPRESSION; LYMPHOID-MYELOID CONVERSION;

D O I：

10.1073/pnas.88.5.1830

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Total nuclear proteins extracted from cell lines representing various stages of differentiation of mouse B lymphocytes were studied by computer analysis of two-dimensional gels. Of the 1438 spots present on the gels, 55 varied significantly in intensity during differentiation. The variations occurred most often in steps correlating with those classically defined for B-cell differentiation. Seventeen spots were not detectable in at least one of the stages (qualitative variations) and could represent switching on or off of genes coding for nuclear proteins. Detailed analysis of the 55 variable spots showed that they fall into small sets characterized by similar expression profiles, which argues for a combinatorial, multistep control mechanism of gene expression. In addition, analysis of the expression of all the nuclear proteins resolved on the gels clearly differentiated B-lineage cells from myeloid cells and suggested that the most important transition in B-cell differentiation occurs between the resting B cell and plasmocyte stages.

引用

页码：1830 / 1834

页数：5

共 32 条

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