THE STRONG BINDING OF LUZOPEPTIN TO DNA

被引：12

作者：

FOX, KR

WOOLLEY, C

机构：

[1] Department of Physiology and Pharmacology, University of Southampton, Southampton, SO9 3TU, Bassett Crescent East

来源：

BIOCHEMICAL PHARMACOLOGY | 1990年 / 39卷 / 05期

基金：

英国惠康基金;

关键词：

D O I：

10.1016/0006-2952(90)90211-3

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

The effect of luzopeptin on the mobility of DNA in polyacrylamide gels has been determined. Experiments on a mixture of DNA fragments of various lengths have shown that the drug does not form intermolecular cross-links. Gel analysis of complexes of the drug with short DNA fragments (15-35 base pairs) reveals a ladder of discrete bands in which each band appears to correspond to the addition of a further drug molecule. The results suggest that luzopeptin binds very strongly to DNA, occupying about four base pairs and displays little or no sequence selectivity. Luzopeptin renders certain adenine residues hyperreactive to diethylpyrocarbonate, these occur in different positions to those affected by echinomycin. © 1990.

引用

页码：941 / 948

页数：8

共 17 条

[11]

McLean M J, 1988, J Mol Recognit, V1, P138, DOI 10.1002/jmr.300010307

[12] HOOGSTEEN BASE-PAIRS PROXIMAL AND DISTAL TO ECHINOMYCIN BINDING-SITES ON DNA [J].

MENDEL, D ;

DERVAN, PB .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1987, 84 (04) :910-914

[13] SEQUENCE-INFLUENCED INTERACTIONS OF OLIGOACRIDINES WITH DNA DETECTED BY RETARDED GEL-ELECTROPHORETIC MIGRATIONS [J].

NIELSEN, PE ;

ZHEN, WP ;

HENRIKSEN, U ;

BUCHARDT, O .

BIOCHEMISTRY, 1988, 27 (01) :67-73

[14] DIETHYL PYROCARBONATE CAN DETECT A MODIFIED DNA-STRUCTURE INDUCED BY THE BINDING OF QUINOXALINE ANTIBIOTICS [J].

PORTUGAL, J ;

FOX, KR ;

MCLEAN, MJ ;

RICHENBERG, JL ;

WARING, MJ .

NUCLEIC ACIDS RESEARCH, 1988, 16 (09) :3655-3670

[15] INTERACTION OF THE ANTITUMOUR ANTIBIOTIC LUZOPEPTIN WITH THE HEXANUCLEOTIDE DUPLEX D(5'-GCATGC)2 - ONE-DIMENSIONAL AND TWO-DIMENSIONAL NMR-STUDIES [J].

SEARLE, MS ;

HALL, JG ;

DENNY, WA ;

WAKELIN, LPG .

BIOCHEMICAL JOURNAL, 1989, 259 (02) :433-441

[16] H-1-NMR AND C-13-NMR STUDIES OF THE ANTITUMOUR ANTIBIOTIC LUZOPEPTIN [J].

SEARLE, MS ;

HALL, JG ;

WAKELIN, LPG .

BIOCHEMICAL JOURNAL, 1988, 256 (01) :271-278

[17] ECHINOMYCIN BINDING-SITES ON DNA [J].

VANDYKE, MM ;

DERVAN, PB .

SCIENCE, 1984, 225 (4667) :1122-1127

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