RICE CELL CRYOPRESERVATION - THE INFLUENCE OF CULTURE METHODS AND THE EMBRYOGENIC POTENTIAL OF CELL-SUSPENSIONS ON POSTTHAW RECOVERY

被引：35

作者：

LYNCH, PT ^{[1
]}

BENSON, EE ^{[1
]}

JONES, J ^{[1
]}

COCKING, EC ^{[1
]}

POWER, JB ^{[1
]}

DAVEY, MR ^{[1
]}

机构：

[1] UNIV NOTTINGHAM,DEPT LIFE SCI,PLANT GENET MANIPULAT GRP,NOTTINGHAM NG7 2RD,ENGLAND

来源：

PLANT SCIENCE | 1994年 / 98卷 / 02期

关键词：

ORYZA SATIVA L CV TAIPEI 309; RICE; CRYOGENIC STORAGE; PROTOPLAST CULTURE; SOMATIC EMBRYOGENESIS; PLANT REGENERATION;

D O I：

10.1016/0168-9452(94)90008-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Protoplast-derived plants were regenerated from cryopreserved embryogenic suspension cultures of the Japonica rice cultivar Taipei 309 at frequencies comparable to those of the original non-frozen cultures. The duration of cryogenic storage did not affect the embryogenic potential of suspension cultures re-established from thawed material. Cells from embryogenic cultures regrew after thawing significantly faster than those from non-embryogenic cultures. The method and frequency of subculture of cells after thawing and the nitrogen status of the recovery medium significantly influenced the rate of cell regrowth. The most rapid post-thaw cell regrowth occurred in cell samples maintained on filter paper overlying semi-solid medium lacking inorganic nitrogen and subcultured 3 days after thawing.

引用

页码：185 / 192

页数：8

共 30 条

[21] REGENERATION OF FERTILE PLANTS FROM PROTOPLASTS OF ELITE INBRED MAIZE [J].

SHILLITO, RD ;

CARSWELL, GK ;

JOHNSON, CM ;

DIMAIO, JJ ;

HARMS, CT .

BIO-TECHNOLOGY, 1989, 7 (06) :581-587

[22] REFINEMENT OF TRIPHENYL TETRAZOLIUM CHLORIDE METHOD OF DETERMINING COLD INJURY [J].

STEPONKUS, PL ;

LANPHEAR, FO .

PLANT PHYSIOLOGY, 1967, 42 (10) :1423-+

[23]

THOMPSON JA, 1986, PLANT SCI, V41, P179

[24]

TOWILL LE, 1990, IN VITRO METHODS CON, P41

[25] DIFFERENCES IN VIABILITY AFTER FREEZE-PRESERVATION IN LIQUID-NITROGEN AMONG RICE CALLUS LINES [J].

WATANABE, K ;

UENO, S ;

MITSUDA, H .

AGRICULTURAL AND BIOLOGICAL CHEMISTRY, 1990, 54 (05) :1295-1296

[26]

WATANABE K, 1992, J PLANT PHYSL, V136, P443

[27]

WITHERS LA, 1980, CRYOLETTERS, V1, P213

[28] PROLINE - NOVEL CRYOPROTECTANT FOR THE FREEZE PRESERVATION OF CULTURED-CELLS OF ZEA-MAYS-L [J].

WITHERS, LA ;

KING, PJ .

PLANT PHYSIOLOGY, 1979, 64 (05) :675-678

[29]

WITHERS LA, 1985, CRYOPRESERVATION PLA, P244

[30]

WITHERS LA, 1979, PHSYL PLANT, V39, P460

← 1 2 3 →