CHARACTERIZATION OF A HUMAN CHROMOSOME-4 FLOW-SORTED COSMID LIBRARY

被引:19
作者
RIESS, O [1 ]
SIEDLACZCK, I [1 ]
KREDTKE, S [1 ]
MELMER, G [1 ]
EPPLEN, JT [1 ]
DEAVEN, LL [1 ]
机构
[1] LOS ALAMOS NATL LAB, LOS ALAMOS, NM 87545 USA
来源
CYTOGENETICS AND CELL GENETICS | 1994年 / 65卷 / 04期
关键词
D O I
10.1159/000133638
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
A cosmid library has been constructed from a hamster x human hybrid cell line and gridded into 270 microtiter plates containing a total of 2 5,920 single colonies. Approximately 84% of the recombinants contain human material, with an average length of 29 kb. This library represents a nearly three-fold coverage of human chromosome 4. We investigated this library for presumptive genes, using a set of oligonucleotides detecting SpI and splice-site consensus sequences. The presence of simple repeat motifs was investigated in the cosmids using the oligonucleotides (GGATTT)3, (GGAT)4, (CAC)5, (GCC)5, (AGC)5, (GATA)4, (GACA)4, and (CA)8 as hybridization probes.
引用
收藏
页码:238 / 242
页数:5
相关论文
共 24 条
  • [11] VARIATION OF THE CGG REPEAT AT THE FRAGILE-X SITE RESULTS IN GENETIC INSTABILITY - RESOLUTION OF THE SHERMAN PARADOX
    FU, YH
    KUHL, DPA
    PIZZUTI, A
    PIERETTI, M
    SUTCLIFFE, JS
    RICHARDS, S
    VERKERK, AJMH
    HOLDEN, JJA
    FENWICK, RG
    WARREN, ST
    OOSTRA, BA
    NELSON, DL
    CASKEY, CT
    [J]. CELL, 1991, 67 (06) : 1047 - 1058
  • [12] CONSTRUCTION OF 15 HUMAN-CHROMOSOME SPECIFIC DNA LIBRARIES FROM FLOW-PURIFIED CHROMOSOMES
    FUSCOE, JC
    CLARK, LM
    VANDILLA, MA
    [J]. CYTOGENETICS AND CELL GENETICS, 1986, 43 (1-2): : 79 - 86
  • [13] ANDROGEN RECEPTOR GENE-MUTATIONS IN X-LINKED SPINAL AND BULBAR MUSCULAR-ATROPHY
    LASPADA, AR
    WILSON, EM
    LUBAHN, DB
    HARDING, AE
    FISCHBECK, KH
    [J]. NATURE, 1991, 352 (6330) : 77 - 79
  • [14] A NOVEL GENE CONTAINING A TRINUCLEOTIDE REPEAT THAT IS EXPANDED AND UNSTABLE ON HUNTINGTONS-DISEASE CHROMOSOMES
    MACDONALD, ME
    AMBROSE, CM
    DUYAO, MP
    MYERS, RH
    LIN, C
    SRINIDHI, L
    BARNES, G
    TAYLOR, SA
    JAMES, M
    GROOT, N
    MACFARLANE, H
    JENKINS, B
    ANDERSON, MA
    WEXLER, NS
    GUSELLA, JF
    BATES, GP
    BAXENDALE, S
    HUMMERICH, H
    KIRBY, S
    NORTH, M
    YOUNGMAN, S
    MOTT, R
    ZEHETNER, G
    SEDLACEK, Z
    POUSTKA, A
    FRISCHAUF, AM
    LEHRACH, H
    BUCKLER, AJ
    CHURCH, D
    DOUCETTESTAMM, L
    ODONOVAN, MC
    RIBARAMIREZ, L
    SHAH, M
    STANTON, VP
    STROBEL, SA
    DRATHS, KM
    WALES, JL
    DERVAN, P
    HOUSMAN, DE
    ALTHERR, M
    SHIANG, R
    THOMPSON, L
    FIELDER, T
    WASMUTH, JJ
    TAGLE, D
    VALDES, J
    ELMER, L
    ALLARD, M
    CASTILLA, L
    SWAROOP, M
    [J]. CELL, 1993, 72 (06) : 971 - 983
  • [15] THE MORBID ANATOMY OF THE HUMAN GENOME - CHROMOSOMAL LOCATION OF MUTATIONS CAUSING DISEASE
    MCKUSICK, VA
    AMBERGER, JS
    [J]. JOURNAL OF MEDICAL GENETICS, 1993, 30 (01) : 1 - 26
  • [16] MELMER G, 1993, IN PRESS ADV ELECTRO
  • [17] Melmer Georg, 1992, Human Molecular Genetics, V1, P433, DOI 10.1093/hmg/1.6.433
  • [18] PARAMETERS OF THE HUMAN GENOME
    MORTON, NE
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1991, 88 (17) : 7474 - 7476
  • [19] AN IMPROVED BACTERIAL COLONY LYSIS PROCEDURE ENABLES DIRECT DNA HYBRIDIZATION USING SHORT (10-BASES, 11-BASES) OLIGONUCLEOTIDES TO COSMIDS
    NIZETIC, D
    DRMANAC, R
    LEHRACH, H
    [J]. NUCLEIC ACIDS RESEARCH, 1991, 19 (01) : 182 - 182
  • [20] RNA SPLICE JUNCTIONS OF DIFFERENT CLASSES OF EUKARYOTES - SEQUENCE STATISTICS AND FUNCTIONAL IMPLICATIONS IN GENE-EXPRESSION
    SHAPIRO, MB
    SENAPATHY, P
    [J]. NUCLEIC ACIDS RESEARCH, 1987, 15 (17) : 7155 - 7174