PRODUCTION, PURIFICATION AND PROPERTIES OF A PICHIA-KLUYVERI KILLER TOXIN

被引:54
作者
MIDDELBEEK, EJ
HERMANS, JMH
STUMM, C
机构
[1] Department of Microbiology, Faculty of Science, University of Nijmegen, Nijmegen
来源
ANTONIE VAN LEEUWENHOEK JOURNAL OF MICROBIOLOGY | 1979年 / 45卷 / 03期
关键词
D O I
10.1007/BF00443282
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Production of the killer toxin of Pichia kluyveri 1002 was stimulated in the presence of yeast extract. In a minimal medium production was optimal at pH 3.8-4.0 and 22-25°C. Addition of gelatin and nonionic detergents, like Brij-58 (polyoxyethylene 20 cetyl ether) and Triton-X-100, to this medium enhanced production significantly. The killer toxin was purified 140-fold by use of a stepwise ethanol precipitation and butyl Sepharose column chromatography. The purified killer toxin, which still contained some carbohydrates, appeared to be glycoprotein with a mol wt of about 19000 and an isoelectric point of 4.3. It was stable between pH 2.5 and 4.7 and up to 40°C. © 1979 H. Veenman & Zonen B. V.
引用
收藏
页码:437 / 450
页数:14
相关论文
共 34 条