INSITU HYBRIDIZATION WITH FLUORESCEINATED DNA

被引：165

作者：

WIEGANT, J

RIED, T

NEDERLOF, PM

VANDERPLOEG, M

TANKE, HJ

RAAP, AK

机构：

[1] Department of Cytochemistry and Cytometry, 2333 AL Leiden

来源：

NUCLEIC ACIDS RESEARCH | 1991年 / 19卷 / 12期

关键词：

D O I：

10.1093/nar/19.12.3237

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have used fluorescein-11-dUTP in a nick-translation format to produce fluoresceinated human nucleic acid probes. After in situ hybridization of fluoresceinated DNAs to human metaphase chromosomes, the detection sensitivity was found to be 50 - 100 kb. The feasibility and the increase in detection sensitivity of microscopic imaging of in situ hybridized, fluoresceinated DNA with an integrating solid state camera for rapid cosmid mapping is illustrated. Combination of fluoresceinated DNA with biotinated and digoxigeninated DNAs allowed easy performance of triple fluorescence in situ hybridization. The potential of these techniques for DNA mapping, cytogenetics and biological dosimetry is briefly discussed.

引用

收藏

页码：3237 / 3241

页数：5

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