COEXPRESSION OF TGF-ALPHA, EPIDERMAL GROWTH-FACTOR RECEPTOR, AND P-GLYCOPROTEIN IN NORMAL AND BENIGN DISEASED BREAST TISSUES

被引：14

作者：

SCALA, S

SAEKI, T

LYNCH, A

SALOMON, D

MERINO, MJ

BATES, SE

机构：

[1] NCI,DIV CANC TREATMENT,CLIN ONCOL PROGRAM,BETHESDA,MD 20892

[2] NCI,TUMOR IMMUNOL & BIOL LAB,TUMOR GROWTH FACTOR SECT,BETHESDA,MD 20892

[3] NCI,PATHOL LAB,BETHESDA,MD 20892

来源：

DIAGNOSTIC MOLECULAR PATHOLOGY | 1995年 / 4卷 / 02期

关键词：

TGF-ALPHA; EGFR; P-GLYCOPROTEIN; BREAST TISSUE; IMMUNOSTAINING;

D O I：

10.1097/00019606-199506000-00010

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Twenty-four normal or benign breast tissues were examined for the expression of transforming growth factor-alpha (TGF alpha), epidermal growth factor receptor (EGFR), and P-glycoprotein, the product of the mdr-1 gene. Specific staining for all three proteins was observed in the majority of the samples. P-glycoprotein staining was present in most (88%), and confined to the lumenal surface of the ductal epithelium. Membranous EGFR expression was observed in epithelial cells in 92% of the specimens and 42% displayed both myoepithelial and epithelial cell staining. TGF alpha staining was intense and uniformly distributed through the cytoplasm (96%). Coexpression of EGFR, TGF alpha, and P-glycoprotein in normal human breast tissues suggests a role for each of those proteins in normal breast physiology. An interaction may be present in normal breast tissue between the EGF receptor pathway and P-glycoprotein.

引用

页码：136 / 142

页数：7

共 28 条

[1] Barrett-Lee P., Travers M., Luqmani Y., Coombes R.C., Transcripts for transforming growth factors in human breast cancer: Clinical correlates, Br J Cancer, 61, pp. 612-617, (1990)
[2] Bates S.E., Davidson N.E., Valverius E.M., Et al., Expression of transforming growth factor-alpha and its messenger ribonucleic acid in human breast cancer: Its regulation by estrogen and its possible functional significance, Mol Endocrinol, 2, pp. 543-555, (1988)
[3] Bates S.E., Lee J.S., Dickstein B., Spolyar M., Fojo A.T., Differential modulation of P-glycoprotein transport by protein kinase inhibition, Biochemistry, 32, pp. 9156-9164, (1993)
[4] Bates S.E., Shieh C.Y., Tsokos M., Expression of mdr-I/P-glycoprotein in human neuroblastoma, Am J Pathol, 139, pp. 305-315, (1991)
[5] Center M.S., Mechanisms regulating cell resistance to adria-mycin. Evidence that drug accumulation in resistant cells is modulated by phosphorylation of a plasma membrane glycoprotein, Biochem Pharmacol, 34, pp. 1471-1476, (1985)
[6] Chambers T.C., Chalikonda I., Eilon G., Correlation of protein kinase C translocation. P-glycoprotein phosphorylation and reduced drug accumulation in multidrug resistant human KB cells, Biochem Biophys Res Comm, 169, pp. 253-269, (1990)
[7] Chan H., Haddad G., Thorner P.S., Et al., P-glycoprotein expression as a predictor of the outcome of therapy for neuroblastoma, N Engl J Med, 325, pp. 1608-1614, (1991)
[8] Cordon-Cardo C., O'brien J.P., Casals D., Et al., Multidrug-resistance gene (P-glycoprotein) is expressed by endothelial cells at blood-brain barrier sites, Proc Natl Acad Sci USA, 86, pp. 695-698, (1989)
[9] Dalton W.S., Grogan T.M., Meltzer P.S., Et al., Drug-resistance in multiple myeloma and non-Hodgkin s lymphoma: Detection of P-glycoprotein and potential circumvention by addition of verapamil to chemotherapy, J Clin Oncol, 4, pp. 415-424, (1989)
[10] Dickstein B., Valverius E.M., Wosikowski K., Et al., Increased EGF receptor in an estrogen-responsive, adriamycin-resistant MCF-7 cell line, J Cell Physiol, 157, pp. 110-118, (1993)

← 1 2 3 →