REGIONS OF THE CFA/I PROMOTER INVOLVED IN THE ACTIVATION BY THE TRANSCRIPTIONAL ACTIVATOR CFAD AND REPRESSION BY THE HISTONE-LIKE PROTEIN H-NS

被引：14

作者：

JORDI, BJAM ^{[1
]}

VANDERZEIJST, BAM ^{[1
]}

GAASTRA, W ^{[1
]}

机构：

[1] UNIV UTRECHT,FAC VET MED,INST INFECT DIS & IMMUNOL,DEPT BACTERIOL,3508 TD UTRECHT,NETHERLANDS

来源：

BIOCHIMIE | 1994年 / 76卷 / 10-11期

关键词：

CFA/I FIMBRIAE; ENTEROTOXIGENIC; ESCHERICHIA COLI; POSITIVE REGULATORS; HISTONE-LIKE PROTEINS;

D O I：

10.1016/0300-9084(94)90029-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Expression of CFA/I fimbriae of Escherichia coli requires the transcriptional activator CfaD. The mechanism by which CfaD activates the CFA/I promoter is to overcome the repression by H-NS, one of the histone-like proteins in E coli. This study addresses the question of which sequences in the promoter region of CFA/I interact with CfaD and H-NS. In order to determine this, deletion mutants of the CFA/I promoter were constructed and cloned upstream of the promoterless lacZ gene. The effect of CfaD and H-NS on the expression of these constructs was determined.

引用

页码：1052 / 1054

页数：3

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