CLONING OF THE CDNA-ENCODING NEURAL ADHESION MOLECULE F3 FROM BOVINE BRAIN

被引：14

作者：

WATANABE, K

SHIMAZAKI, K

HOSOYA, H

FUKAMAUCHI, F

TAKENAWA, T

机构：

[1] JICHI MED SCH, DEPT PHYSIOL, MINAMI KAWACHI, TOCHIGI 32904, JAPAN

[2] TOKYO MED & DENT UNIV, MED RES INST, DEPT MOLEC MED SCI, CHIYODA KU, TOKYO 101, JAPAN

[3] UNIV TOKYO, INST MED SCI, DEPT MOLEC ONCOL, MINATO KU, TOKYO 108, JAPAN

来源：

GENE | 1995年 / 160卷 / 02期

关键词：

F11; CONTACTIN; IG SUPERFAMILY; GPI ANCHOR; CELL ADHESION; BRAIN FUNCTION;

D O I：

10.1016/0378-1119(95)00062-B

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

We cloned a bovine cDNA encoding the neural adhesion molecule F3 and analyzed its nucleotide sequence. The coding region consisted of 3054 bp encoding 1018 amino acid (aa) residues. The M(r) calculated from the deduced aa sequence was 113 383. Bovine F3 had 93, 94 and 77% aa identity with the mouse, human and chicken homologs, respectively. Bovine F3, similar to those of chicken and human, was devoid of two aa residues (Ile-Thr) in the sixth immunoglobulin type C2-like domain, as compared with the mouse homolog. Parts of bovine F3 protein were overproduced in Escherichia coli. The antibodies raised against the recombinant proteins in rabbits reacted specifically with F3. F3 protein was detected in cerebellum cerebrum and spinal cord in Western blot analysis.

引用

页码：245 / 248

页数：4

共 21 条

[1] SEROTONIN-MEDIATED ENDOCYTOSIS OF APCAM - AN EARLY STEP OF LEARNING-RELATED SYNAPTIC GROWTH IN APLYSIA [J].