APPLICATION OF HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY AND GAS CHROMATOGRAPHY-MASS SPECTROMETRY TO ANALYSIS OF PROSTAGLANDIN-E1 IN BIOLOGICAL MEDIA

被引：20

作者：

WHORTON, AR

SWEETMAN, BJ

OATES, JA

机构：

[1] Department of Pharmacology, Vanderbilt University Medical Center, Nashville

来源：

ANALYTICAL BIOCHEMISTRY | 1979年 / 98卷 / 02期

基金：

美国国家卫生研究院;

关键词：

D O I：

10.1016/0003-2697(79)90167-2

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A method for the quantitation of prostaglandin (PG) E1 in biological samples of gas chromatography-mass spectrometry has been developed. PGE1 was separated from PGE2, 13,14-dihydro-PGE2, and other potentially interfering prostaglandins by reversed phase high performance liquid chromatography. After conversion of PGE1 to PGB1 by treatment with methanolic KOH, PGB1 was derivatized to the methyl ester trimethylsilyl ether and analyzed by selected ion monitoring using hexadeutero-PGE1 as an internal standard. Measurable levels of PGE1 were found in human and rat urine and in incubates of rat and rabbit renal papilla. PGE1 excretion and production by renal slices was blocked by treatment with indomethacin. A complete mass spectrum of derivatized PGE1 was obtained from PGE1 generated by rabbit renal papillary slices. © 1979.

引用

页码：455 / 463

页数：9

共 15 条

[11] QUANTITATIVE GAS CHROMATOGRAPHY OF PROSTAGLANDIN-E1 AT NANOGRAM LEVEL - USE OF DEUTERATED CARRIER AND MULTIPLE-ION ANALYZER [J].