HYDROGEN-PEROXIDE OXYGEN BIOSENSOR BASED ON SUPPORTED PHOSPHOLIPID-BILAYER

被引：42

作者：

OTTO, M

SNEJDARKOVA, M

REHAK, M

机构：

[1] Institute of Animal Biochemistry and Genetics, Slovak Academy of Sciences, 900 28 Ivanka Dunaji, Czechoslovakia

来源：

ANALYTICAL LETTERS | 1992年 / 25卷 / 04期

关键词：

BIOSENSOR; BLACK LIPID MEMBRANE; HYDROGEN PEROXIDE; OXYGEN;

D O I：

10.1080/00032719208020025

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The influence of lipid concentration, lipid composition, pH and temperature on the stability and function of metal-supported phospolipid bilayer membranes (s-BLM) was studied and the potential applicability of this system in biosensor development was explored. Parameters to be measured were the resistance as well as the amperometric response of the system to oxygen and hydrogen peroxide. Stability and function of the s-BLM system are inversely related to the lipid concentration. Cholesterol exerts a stabilizing effect at a molar ratio of 4:1 to 1:3 (PC : cholesterol). The effect of stearylamine on membrane stability depends on the sign of the polarizing voltage. Ascorbic acid, but not citric acid nor glutathion nor uric acid interferes with measurements of hydrogen peroxide. The s-BLM is remarkably stable in urine. Its use as a simple but sensitive oxygen sensor for measuring the respiration rate of rat liver mitochondria is demonstrated.

引用

页码：653 / 662

页数：10

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