EFFECT OF OKADAIC ACID ON HUMAN BASOPHIL SECRETION

被引：13

作者：

BOTANA, LM ^{[1
]}

MACGLASHAN, DW ^{[1
]}

机构：

[1] JOHNS HOPKINS ASTHMA & ALLERGY CTR,5501 HOPKINS BAYVIEW CIRCLE,BALTIMORE,MD 21224

来源：

BIOCHEMICAL PHARMACOLOGY | 1993年 / 45卷 / 11期

关键词：

D O I：

10.1016/0006-2952(93)90204-A

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

We examined the effects of a phosphatase inhibitor, okadaic acid, on mediator secretion from human basophils. These cells are known to respond to a number of stimuli that appear to utilize distinct biochemical pathways converging on mediator release. Okadaic acid was found to inhibit IgE-mediated release (histamine release inhibited 80 +/- 12% and leukotriene release inhibited 100% following a 10-min preincubation with okadaic acid and stimulation with an optimal concentration of anti-IgE antibody) at a concentration of 1 uM. The concentration-response curve to okadaic acid was steep, with 0.1 muM yielding only 20 +/- 10% inhibition of either mediator. Secretion following stimulation with the univalent stimulus, fMet-Leu-Phe peptide, was not inhibited by okadaic acid. Unlike cAMP-active agents that inhibit cytosolic Ca2+ elevations following IgE-mediated stimulation, the increased state of cellular protein phosphorylation, which presumably results from treatment with 1 muM okadaic acid, had no effect on the elevations in free cytosolic Ca2+ that follow stimulation with anti-IgE antibody of fMet peptide.

引用

页码：2311 / 2315

页数：5

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