STRUCTURAL CHARACTERIZATION OF LIPID-A COMPONENT OF ERWINIA-CAROTOVORA LIPOPOLYSACCHARIDE

被引:17
作者
FUKUOKA, S
KAMISHIMA, H
NAGAWA, Y
NAKANISHI, H
ISHIKAWA, K
NIWA, Y
TAMIYA, E
KARUBE, I
机构
[1] GOVT IND RES INST,SHIKO KU,TAKAMATSU 761,JAPAN
[2] NATL CHEM LAB IND,TSUKUBA 305,JAPAN
[3] UNIV TOKYO,ADV SCI & TECHNOL RES CTR,MEGURO KU,TOKYO 153,JAPAN
关键词
ERWINIA CAROTOVORA; LIPOPOLYSACCHARIDE; LIPID-A STRUCTURE; COMPONENT ANALYSIS; FAB-MS; 2D-NMR;
D O I
10.1007/BF00248674
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The chemical structure of the lipid A component of lipopolysaccharide excreted into the liquid medium by the plant pathogenic enterobacterium Erwinia carotovora FERM P-7576 was characterized. It consists of a beta-1', 6-linked glucosamine disaccharide which carries ester- and amide-bound fatty acids and phosphate similar to the lipid A from other gram-negative bacteria. The lipid A preparation was not uniform in the number and composition of the fatty acids linked to the disaccharide. Four prominent lipids A were involved, they were composed of five to seven residues of fatty acid. Among them the major component was hexa-acyl lipid A, in which the hydroxyl group at position 3' and the amino group of the non-reducing glucosamine unit carry 3-dodecanoyl-oxytetradecanoyl residues. Positions 2 and 3 of the reducing glucosamine unit were substituted by 3-hydroxytetradecanoic acid. In the hepta-acyl lipid A, an additional hexadecanoic acid was linked to the hydroxyl group of the 3-hydroxytetradecanoyl residue at position 2 of the hexa-acyl lipid A. Two penta-acyl lipids A were the homologs of the hexa-acyl lipid A with decreasing acylation. Dodecanoic acid was missing from one, and 3-hydroxytetradecanoic acid from another. 3-Dodecanoyloxytetradecanoyl residue at position 3' differentiates E. carotovora lipid A from that of other gram-negative bacteria.
引用
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页码:311 / 318
页数:8
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