SOLID-PHASE PURIFICATION IN AUTOMATED DNA-SEQUENCING

被引：12

作者：

TONG, XC ^{[1
]}

SMITH, LM ^{[1
]}

机构：

[1] UNIV WISCONSIN,DEPT CHEM,MADISON,WI 53706

来源：

DNA SEQUENCE | 1993年 / 4卷 / 03期

关键词：

AUTOMATED DNA SEQUENCING; BIOTIN; FLUORESCENCE DUPLICATION SOLID PHASE PURIFICATION; MAGNETIC PARTICLES; STREPTAVIDIN;

D O I：

10.3109/10425179309015628

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A solid phase purification method has been utilized to investigate the effect of template DNA and other contaminants present in enzymatic DNA sequencing reactions upon data quality in automated DNA sequence analysis. The primer oligonucleotide is prepared with an internal biotin group and one of four fluorescent tags at the 5' terminus. The fluorescently labeled DNA fragments produced in the sequencing reactions are separated from other reaction components, including template DNA, using superparamagnetic streptavidin beads. Template DNA and other reaction components may then be added back to the purified material to assess their effect upon sequence data. It is found that band intensity and base calling accuracy are decreased by the presence of the template DNA and other reaction components.

引用

页码：151 / 162

页数：12

共 23 条

[1]

COTTON FA, 1980, ADV INORGANIC CHEM

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