HIGH-SPEED DNA SEQUENCING BY CAPILLARY ELECTROPHORESIS

被引：198

作者：

LUCKEY, JA ^{[1
]}

DROSSMAN, H ^{[1
]}

KOSTICHKA, AJ ^{[1
]}

MEAD, DA ^{[1
]}

DCUNHA, J ^{[1
]}

NORRIS, TB ^{[1
]}

SMITH, LM ^{[1
]}

机构：

[1] UNIV WISCONSIN,DEPT CHEM,MADISON,WI 53706

来源：

NUCLEIC ACIDS RESEARCH | 1990年 / 18卷 / 15期

关键词：

D O I：

10.1093/nar/18.15.4417

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A major challenge of the Human Genome Initiative is the development of a rapid, accurate, and efficient DNA sequencing technology. A major limitation of current technology is the relatively long time required to perform the gel electrophoretic separations of DNA fragments produced in the sequencing reactions. We demonstrate here that it is possible to increase the speed of sequence analysis by over an order of magnitude by performing the electrophoresis and detection in ultra thin capillary gels. An instrument which utilizes these high speed separations to simultaneously analyze many samples will constitute a second generation automated DNA sequencer suitable for large-scale sequence analysis. © 1990 Oxford University Press.

引用

页码：4417 / 4421

页数：5

共 18 条

[1] SUBATTOMOLE AMINO-ACID ANALYSIS BY CAPILLARY ZONE ELECTROPHORESIS AND LASER-INDUCED FLUORESCENCE [J].