GALACTOSYLATION AND GLUCOSYLATION BY USE OF BETA-GALACTOSIDASE

被引：51

作者：

SAUERBREI, B ^{[1
]}

THIEM, J ^{[1
]}

机构：

[1] UNIV HAMBURG,INST ORGAN CHEM,MARTIN LUTHER KING PL 6,W-2000 HAMBURG 13,GERMANY

来源：

TETRAHEDRON LETTERS | 1992年 / 33卷 / 02期

关键词：

D O I：

10.1016/0040-4039(92)88050-F

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

The transglycosylation activity of beta-galactosidase derived from Aspergillus oryzae and Escherichia coli, respectively, was examined in reaction systems containing up to 50% acetonitrile. Starting with ortho-nitrophenyl beta-galactoside (1), which functions both as donor and as acceptor, beta-Gal(1-6)beta-Gal-PhNO2-omicron (2) and beta-Gal(1-3)beta-Gal-PhNO2-omicron (3) were obtained. Under similar conditions the enzyme from A. oryzae converts para-nitrophenyl beta-glucoside (5) to beta-Glc(1-2)beta-Glc-PhNO2-p (6) and alpha-Glc(1-4)beta-Glc-PhNO2-p (7). Incubation of 1 and L-serine in the presence of the A. oryzae beta-galactosidase leads to beta-Gal-L-Ser (4).

引用

页码：201 / 204

页数：4

共 17 条

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