A 36-KDA MITOCHONDRIAL PROTEIN IS RESPONSIBLE FOR CYANIDE-RESISTANT RESPIRATION IN HANSENULA-ANOMALA

被引：26

作者：

MINAGAWA, N

SAKAJO, S

KOMIYAMA, T

YOSHIMOTO, A

机构：

[1] Department of Biochemistry, Niigata College of Pharmacy, Niigata, 950-21

来源：

FEBS LETTERS | 1990年 / 264卷 / 01期

关键词：

Alternative oxidase; Cyanide-resistant respiration; Mitochondria; Precursor; Protein import;

D O I：

10.1016/0014-5793(90)80787-J

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Antimycin A-dependent induction of cyanide-resistant respiration in Hansenula anomala was reversibly blocked by carbonylcyanide-m-chlorophenylhydrazone (CCCP). When the cells were pulse-labeled with [35S]methionine in the presence of both antimycin A and CCCP, the radioactivity was incorporated into a 39 kDa mitochondrial protein. Upon removal of CCCP, this protein was processed into a 36 kDa form. The increase in the 36 kDa protein completely paralleled that in cyanide-resistant respiration activity, suggesting that the 39 kDa protein is the precursor of the 36 kDa protein, which is responsible for cyanide-resistant respiration. © 1990.

引用

页码：149 / 152

页数：4

共 12 条

[1] DETERMINATION OF MOLECULAR MASS OF THE AROID ALTERNATIVE OXIDASE BY RADIATION-INACTIVATION ANALYSIS [J].