CDC39, AN ESSENTIAL NUCLEAR-PROTEIN THAT NEGATIVELY REGULATES TRANSCRIPTION AND DIFFERENTIALLY AFFECTS THE CONSTITUTIVE AND INDUCIBLE HIS3 PROMOTERS

The yeast HIS3 promoter region contains two functionally distinct TATA elements, T(C) and T(R), that are responsible respectively for initiation from the +1 and +13 sites. Both T(C) and T(R) support basal HIS3 transcription and require the TATA binding protein TFIID, but only T(R) responds to transcriptional activation by GCN4 and GAL4. By selecting for yeast strains that increase transcription by a GCN4 derivative with a defective activation domain, we have isolated a temperature-sensitive mutation in CDC39, a previously defined gene implicated in cell-cycle control and the pheromone response. This cdc39-2 mutation causes increased basal transcription of many, but not all genes, as well as increased transcriptional activation by GCN4 and GAL4. Surprisingly, basal HIS3 transcription from the +1 initiation site is strongly increased, while initiation from the +13 site is barely affected. Thus, unlike acidic activator proteins that function through T(R), CDC39 preferentially affects transcription mediated by T(C). CDC39 is an essential gene that encodes a very large nuclear protein (2108 amino acids) containing two glutamine-rich regions. These observations suggest that CDC39 negatively regulates transcription either by affecting the general RNA polymerase II machinery or by altering chromatin structure.