FRUCTOSE PRODUCTION - COIMMOBILIZED AMYLOGLUCOSIDASE, PULLULANASE, AND GLUCOSE-ISOMERASE ON BIOBONE(TM)

被引：9

作者：

SCHAFHAUSER, DY

STOREY, KB

机构：

[1] CARLETON UNIV,INST BIOCHEM,DEPT BIOL,OTTAWA K1S 5B6,ONTARIO,CANADA

[2] CARLETON UNIV,INST BIOCHEM,DEPT CHEM,OTTAWA K1S 5B6,ONTARIO,CANADA

来源：

APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY | 1992年 / 36卷 / 01期

关键词：

AMYLOGLUCOSIDASE; CHICKEN BONE; PULLULANASE; ENZYME COIMMOBILIZATION; GLUCOSE ISOMERASE; HIGH FRUCTOSE SYRUP;

D O I：

10.1007/BF02950775

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Amyloglucosidase, pullulanase, and glucose isomerase were coimmobilized onto granular chicken bone (BIOBONE(TM)). Enzyme ratios showing optimum glucose and fructose production (0.7:10:22.3 U amyloglucosidase: pullulanase: glucose isomerase) resulted in 14.4 +/- 1.9% of activity bound relative to an equal amount of free enzyme. The estimated specific activity for these enzymes decreased 4.6-fold with immobilization. Reaction pH strongly influenced the yield and ratio of glucose and fructose produced. Net hexose production from the immobilized system was optimal at pH 6.5 and 55-degrees-C with a fructose yield of about 20%.

引用

页码：63 / 74

页数：12