ALTERED SUBSTRATE SELECTIVITY OF PKC-ETA PSEUDOSUBSTRATE SITE MUTANTS

被引：20

作者：

DEKKER, LV ^{[1
]}

MCINTYRE, P ^{[1
]}

PARKER, PJ ^{[1
]}

机构：

[1] SANDOZ INST MED RES, LONDON WC1E 6BN, ENGLAND

来源：

FEBS LETTERS | 1993年 / 329卷 / 1-2期

关键词：

PROTEIN KINASE-C; PROTEIN KINASE-C-ETA; PHOSPHORYLATION; PSEUDOSUBSTRATE;

D O I：

10.1016/0014-5793(93)80208-C

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Three protein kinase C (PKC)-eta mutants were constructed in which the whole or part of the pseudosubstrate site was replaced with corresponding parts of the PKC-alpha pseudosubstrate site. The resulting chimaeric kinases were compared with wild-type PKC-eta in their ability to phosphorylate a PKC-eta peptide substrate or histone. Changes in the pseudosubstrate site of PKC-eta are accompanied by changes in substrate selectivity, indicating that the substrate selectivity observed for PKC-eta is at least in part due to its pseudosubstrate site.

引用

页码：129 / 133

页数：5

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