LEISHMANIA (VIANNIA) LAINSONI OCCUPIES A UNIQUE NICHE WITHIN THE SUBGENUS VIANNIA

被引:17
作者
FRESH, S [1 ]
DEBRUIJN, MHL [1 ]
MENDOZALEON, JA [1 ]
BARKER, DC [1 ]
机构
[1] UNIV CAMBRIDGE,DEPT PATHOL,MOLTENO LABS,NATL INST MED RES,MRC OUTSTN,CAMBRIDGE CB2 1QP,ENGLAND
关键词
LEISHMANIA LAINSONI; CHARACTERIZATION; NUCLEAR DNA; KDNA; PCR;
D O I
10.1016/0035-9203(95)90509-X
中图分类号
R1 [预防医学、卫生学];
学科分类号
1004 ; 120402 ;
摘要
Leishmania lainsoni has recently been recognized as a new peripylarian species belonging to the subgenus Viannia and the L. braziliensis complex. It has been isolated from its sandfly vector, reservoir host and cutaneous lesions of human patients. Microscopical examination has shown characteristics which are different from those of other members of the L. braziliensis complex. Nuclear deoxyribonucleic acid (DNA) hybridization patterns with a beta tubulin probe and kinetoplast DNA buoyant density measurements show close similarities with other species of the L. braziliensis complex. However, kinetoplast DNA restriction enzyme fragment patterns of L. (V.) lainsoni isolates show similarities to L. mexicana complex species as well as weak cross hybridization. L. (V.) lainsoni is also amplified with L. braziliensis complex specific polymerase chain reaction (PCR) primers but it requires a lower annealing temperature and gives a 300 base pair PCR product. A possible model for the binding of PCR primers to the L. (V.) lainsoni kinetoplast DNA minicircle is proposed.
引用
收藏
页码:231 / 236
页数:6
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