RAPID IDENTIFICATION OF STAPHYLOCOCCUS-SAPROPHYTICUS USING AN OLIGONUCLEOTIDE PROBE COMPLEMENTARY TO 16S RIBOSOMAL-RNA

被引：1

作者：

GASZEWSKAMASTALARZ, A ^{[1
]}

ZAKRZEWSKACZERWINSKA, J ^{[1
]}

MORDARSKI, M ^{[1
]}

机构：

[1] POLISH ACAD SCI,INST IMMUNOL & EXPTL THERAPY,PL-53114 WROCLAW,POLAND

来源：

SYSTEMATIC AND APPLIED MICROBIOLOGY | 1995年 / 18卷 / 01期

关键词：

STAPHYLOCOCCUS SAPROPHYTICUS; 16S RIBOSOMAL-RNA; OLIGONUCLEOTIDE PROBE;

D O I：

10.1016/S0723-2020(11)80459-5

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The complete nucleotide sequence of the 16S rRNA gene of Staphylococcus saprophyticus, one of the most frequently encountered clinically significant members of the coagulase-negative staphylococci, was determined. An oligonucleotide probe (pSs) was defined for the identification of S. saprophyticus by comparing the sequences of the 16S rRNA genes of numerous representative staphylococci. A primer-specific PCR was tested to differentiate S. epidermidis and S. saprophyticus without the need for hybridization.

引用

页码：123 / 126

页数：4

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