COEXPRESSION OF TYPE-I AND TYPE-II IL-1 RECEPTORS IN THE MURINE-T HELPER 2 CELL-LINE D10N

被引：8

作者：

BRIGELIUS-FLOHE, R ^{[1
]}

MCCARTHY, JEG ^{[1
]}

RESCH, K ^{[1
]}

SZAMEL, M ^{[1
]}

MARTIN, M ^{[1
]}

机构：

[1] GBF, BRAUNSCHWEIG, GERMANY

来源：

FEBS LETTERS | 1993年 / 326卷 / 1-3期

关键词：

INTERLEUKIN-1; RECEPTOR; INTERLEUKIN-1 RECEPTOR ANTAGONIST; MOUSE-T HELPER CELL;

D O I：

10.1016/0014-5793(93)81791-W

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

IL-1 receptor heterogeneity in murine lymphocytes was investigated by cross-linking to [I-125]IL-1alpha and competition with IL-1 receptor antagonist, and the molecular identity of the IL-1 receptors was identified with PCR using primers specific for type I and type II IL-1 receptors. The thymoma cell line EI4 6.1 exhibits exclusively the 80 kDa receptors which proved to be the type I receptor according to PCR analysis. In the pre-B cell fine 70Z/3, predominantly a 60 kDa type II receptor but also a trace of type I receptor can be identified by PCR. The Th2 cell line D10N expresses both types of IL-1 receptors in equivalent amounts according to cross-linking experiments and PCR. The proliferative response of D10N cells to IL-1 is inhibited by IL-1 ra which according to cross-linking affects the binding to the type I receptor only. It is concluded that coexpression of both types of IL-1 receptors might be a characteristic of murine Th2 cells and that their growth-dependence on IL-1 is mediated by the type I receptor.

引用

页码：204 / 208

页数：5

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