EFFECT OF TETRACYCLINES WHICH HAVE METALLOPROTEINASE INHIBITORY CAPACITY ON BASAL AND HEPARIN-STIMULATED BONE-RESORPTION BY CHICK OSTEOCLASTS

被引:16
作者
CHOWDHURY, MH
MOAK, SA
RIFKIN, BR
GREENWALD, RA
机构
[1] LONG ISL JEWISH MED CTR,ALBERT EINSTEIN COLL MED,DIV RHEUMATOL,LONG ISL CAMPUS,NEW HYDE PK,NY 11042
[2] NYU,SCH DENT MED,NEW YORK,NY 10003
来源
AGENTS AND ACTIONS | 1993年 / 40卷 / 1-2期
关键词
D O I
10.1007/BF01976761
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
Several tetracyclines (TETs) are potent inhibitors of collagenase (CGase) and can inhibit connective tissue degradation in a variety of inflammatory and degenerative disorders. The role of CGase in bone resorption by osteoclasts (OC) remains unclear. Disaggregated OCs from chick embryos were cultured for 24 h on devitalized bovine cortical bone + heparin in the presence of various TETs. Doxycycline (Dox) inhibited pit formation in a dose-dependent manner. CMT, a TET derivative which inhibits matrix metalloproteinases (MMPs) but is not antimicrobial, also inhibited chick OC bone resorption. Heparin markedly stimulated bone resorption at 5 mug/ml, which was reversed by Dox, 5 mug/ml. TETs can reversibly inhibit both basal and heparin-stimulated bone resorption by chick OCs. These findings suggest that MMPs may play a role in osteoclastic bone resorption, and that safe and effective inhibitors of MMPs, including certain TETs, might have a potential therapeutic role.
引用
收藏
页码:124 / 128
页数:5
相关论文
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