DETERMINATION OF PARAQUAT IN FRUITS AND VEGETABLES BY A MAGNETIC PARTICLE BASED ENZYME-LINKED-IMMUNOSORBENT-ASSAY

被引：29

作者：

SELISKER, MY ^{[1
]}

HERZOG, DP ^{[1
]}

ERBER, RD ^{[1
]}

FLEEKER, JR ^{[1
]}

ITAK, JA ^{[1
]}

机构：

[1] N DAKOTA STATE UNIV,DEPT BIOCHEM,FARGO,ND 58105

来源：

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 1995年 / 43卷 / 02期

关键词：

PARAQUAT; ELISA; IMMUNOASSAY; ACID EXTRACTION;

D O I：

10.1021/jf00050a053

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

A competitive enzyme-linked immunosorbent assay (ELISA) for the quantitation of the free paraquat cation was developed using polyclonal rabbit anti-paraquat antibodies bound to a magnetic particle solid phase. A method for the extraction of the paraquat cation from various fruits and vegetables in less than 30 min was developed for use in conjunction with the immunoassay. The procedure has an estimated detection limit of 10 ng/g (ppb) paraquat cation based on the 20 pg/mL estimate in buffer. Results can be easily converted to the dichloride result by dividing the cation concentration by 0.724. Paraquat cation recoveries averaged 99% using this extraction method and immunoassay for cabbage, apples, and potatoes. A collaborative study in which paraquat-fortified zucchini samples were extracted using the traditional 5 h reflux prior to spectrophotometric and immunoassay measurement yielded a correlation of 0.994 with a slope of 1.05.

引用

页码：544 / 547

页数：4

共 17 条

[1] BOOKBINDER MJ, 1986, CLIN CHEM, V32, P1734
[2] CHICHILA TM, 1991, J ASSOC OFF ANA CHEM, V74, P961
[3] DEVELOPMENT OF AN ENZYME-LINKED-IMMUNOSORBENT-ASSAY FOR CAFFEINE
FICKLING, SA
HAMPTON, SM
TEALE, D
MIDDLETON, BA
MARKS, V
[J]. JOURNAL OF IMMUNOLOGICAL METHODS, 1990, 129 (02) : 159 - 164
[4] Hamish K., 1991, AGROCHEMICALS HDB
[5] Hayes WJ, 1991, HDB PESTICIDE TOXICO
[6] DETERMINATION OF BENOMYL (AS CARBENDAZIM) AND CARBENDAZIM IN WATER, SOIL, AND FRUIT JUICE BY A MAGNETIC PARTICLE-BASED IMMUNOASSAY
ITAK, JA
SELISKER, MY
JOURDAN, SW
FLEEKER, JR
HERZOG, DP
[J]. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 1993, 41 (12) : 2329 - 2332
[7] ITAK JA, 1994, J AOAC INT, V77, P86
[8] LARENCE JF, 1981, HIGH PERFORMANCE LIQ, P211
[9] RUBIO F M, 1991, Food and Agricultural Immunology, V3, P113, DOI 10.1080/09540109109354738
[10] A SEQUENTIAL SPECTROPHOTOMETRIC METHOD FOR THE DETERMINATION OF PARAQUAT AND DIQUAT IN PLASMA
SELLERO, IS
LOPEZRIVADULLA, M
CRUZ, A
BERMEJO, A
FERNANDEZ, P
[J]. ANALYTICAL LETTERS, 1993, 26 (09) : 1891 - 1904

← 1 2 →