FUNCTIONAL-CHARACTERIZATION AND TRANSCRIPTIONAL ANALYSIS OF A GENE-CLUSTER GOVERNING EARLY AND LATE STEPS IN DAUNORUBICIN BIOSYNTHESIS IN STREPTOMYCES-PEUCETIUS

被引：61

作者：

MADDURI, K

HUTCHINSON, CR

机构：

[1] UNIV WISCONSIN,SCH PHARM,MADISON,WI 53706

[2] UNIV WISCONSIN,DEPT BACTERIOL,MADISON,WI 53706

来源：

JOURNAL OF BACTERIOLOGY | 1995年 / 177卷 / 13期

关键词：

D O I：

10.1128/jb.177.13.3879-3884.1995

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Sequence analysis of the Streptomyces peucetius daunorubicin biosynthetic gene cluster revealed a partial (dnrQ) and two complete (dnrD and dnrP) open reading frames flanking dnrK. Bioconversion experiments showed that DnrD converts aklanonic acid methylester to aklaviketone and that DnrC is a methyltransferase that converts aklanonic acid to aklanonic acid methylester. The deduced dnrP gene product, homologous to known esterases, may catalyze the conversion of 10-carbomethoxy-13-deoxycarminomycin to its 10-carboxy derivative. The dnrKPQS genes may be transcribed as a polycistronic mRNA.

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页码：3879 / 3884

页数：6

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