FUNCTIONAL-CHARACTERIZATION AND TRANSCRIPTIONAL ANALYSIS OF A GENE-CLUSTER GOVERNING EARLY AND LATE STEPS IN DAUNORUBICIN BIOSYNTHESIS IN STREPTOMYCES-PEUCETIUS

被引:61
作者
MADDURI, K
HUTCHINSON, CR
机构
[1] UNIV WISCONSIN,SCH PHARM,MADISON,WI 53706
[2] UNIV WISCONSIN,DEPT BACTERIOL,MADISON,WI 53706
关键词
D O I
10.1128/jb.177.13.3879-3884.1995
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Sequence analysis of the Streptomyces peucetius daunorubicin biosynthetic gene cluster revealed a partial (dnrQ) and two complete (dnrD and dnrP) open reading frames flanking dnrK. Bioconversion experiments showed that DnrD converts aklanonic acid methylester to aklaviketone and that DnrC is a methyltransferase that converts aklanonic acid to aklanonic acid methylester. The deduced dnrP gene product, homologous to known esterases, may catalyze the conversion of 10-carbomethoxy-13-deoxycarminomycin to its 10-carboxy derivative. The dnrKPQS genes may be transcribed as a polycistronic mRNA.
引用
收藏
页码:3879 / 3884
页数:6
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