CLONING AND SEQUENCE-ANALYSIS OF AN ESTERASE GENE FROM PSEUDOMONAS SP KWI-56

被引：15

作者：

SHIMADA, Y

NAGAO, T

SUGIHARA, A

IIZUMI, T

YUI, T

NAKAMURA, K

FUKASE, T

TOMINAGA, Y

机构：

[1] KURITA WATER IND LTD,KURITA CENT LAB,KANAGAWA,JAPAN

[2] MIYAZAKI UNIV,FAC ENGN,DEPT MAT SCI,MIYAZAKI 880,JAPAN

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1993年 / 1174卷 / 01期

关键词：

ESTERASE; NUCLEOTIDE SEQUENCE; CLONING; SEQUENCE ANALYSIS; (PSEUDOMONAS);

D O I：

10.1016/0167-4781(93)90095-U

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The gene encoding an esterase from Pseudomonas sp. KWI-56 was cloned and sequenced. The nucleotide sequence contained an open reading frame encoding a polypeptide comprising 262 amino acids, whose molecular weight agreed well with the value obtained by SDS-PAGE. Comparison of the amino acid sequence with those of the other homologous enzymes suggested that Ser-92 and His-241 might be included in the catalytic triad.

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页码：79 / 82

页数：4

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