OPTIMIZATION OF INDIRECT PHOTOMETRIC DETECTION OF ANIONS IN HIGH-PERFORMANCE CAPILLARY ELECTROPHORESIS

Optimization of the indirect photometric detection in high-performance capillary electrophoresis is demonstrated. The influences of background electrolyte (BGE) concentration, capillary diameters, linear polymers, pH of BGE and types of BGE have been studied and assessed in terms of theoretical predictions. The experimental results fit the theoretical deduction very well. At optimized conditions, sub-femtomoles of simple anions can be detected, offering a 500-fold improvement compared to previous studies, and being close to the predicted limit of detection.