DETECTION OF HERBICIDES VIA A BACTERIAL PHOTOREACTION CENTER AND BACTERIAL LUCIFERASE

被引：7

作者：

JOCKERS, R ^{[1
]}

SCHMID, RD ^{[1
]}

机构：

[1] GESELL BIOTECHNOL FORSCH MBH,BIOSENSORS PROJECT GRP,W-3300 BRAUNSCHWEIG,GERMANY

来源：

BIOSENSORS & BIOELECTRONICS | 1993年 / 8卷 / 06期

关键词：

PS-II HERBICIDES; BACTERIAL LUCIFERASE; REACTION CENTER; ALDEHYDES;

D O I：

10.1016/0956-5663(93)85008-C

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

A new detection principle for ligands (here photosystem-II herbicides) based on bacterial luciferase as the detection system is presented. The dependency of this enzyme on long-chain aliphatic aldehydes was harnessed. The requirements for such a system are investigated. Binding properties of artificial aldehydes to the Q(B)-binding site of photoreaction centres from the phototrophic bacteria Rhodabacter sphaeroides and substrate properties for bacterial luciferase from Photobacterium fischeri of various artificial aldehydes were determined.

引用

页码：281 / 289

页数：9