SEQUENCE AND CHARACTERIZATION OF MUTT FROM PROTEUS-VULGARIS

被引：39

作者：

KAMATH, AV ^{[1
]}

YANOFSKY, C ^{[1
]}

机构：

[1] STANFORD UNIV,MED CTR,SCH MED,DEPT BIOL SCI,STANFORD,CA 94305

来源：

GENE | 1993年 / 134卷 / 01期

关键词：

RECOMBINANT DNA; TRYPTOPHANASE; MUTATOR GENE; TNA-MUTT REARRANGEMENT;

D O I：

10.1016/0378-1119(93)90180-B

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

A cloned DNA fragment containing the tryptophanase (tna) operon of Proteus vulgaris was found to contain a gene analogous to mutT of Escherichia coli immediately distal to the tna operon. The presumptive mutT of P. vulgaris was shown to be a functional gene by complemention of a mutT mutant from E. coli. The deduced amino acid sequence of the MutT polypeptide of P. vulgaris was 47% identical and 70% similar to MutT of E. coli. The mutT and tna operons of P. vulgaris were shown to be adjacent on the genome of this organism. These operons are located about 20 min apart in the E. coli genome. Our findings suggest that either or both tna and mutT have different genomic locations in the two organisms.

引用

页码：99 / 102

页数：4

共 17 条

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