STAINING METHODS IN GEL-ELECTROPHORESIS, INCLUDING THE USE OF MULTIPLE DETECTION METHODS

被引:59
作者
WIRTH, PJ
ROMANO, A
机构
[1] Biopolymer Chemistry Section, Laboratory of Experimental Carcinogenesis, National Cancer Institute, Bethesda, MD 20892, Building 37
关键词
D O I
10.1016/0021-9673(94)00879-E
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Polyacrylamide gel electrophoresis is a reliable and widely used technique for the separation, identification and characterization of proteins and protein mixtures. With the introduction of high resolution two-dimensional polyacrylamide gel electrophoresis in 1975 upward to 2000 individual polypeptides spots are easily separated on a single electrophoretic gel thereby necessitating the availability of highly sensitive protein detection methods. Although a plethora of protein-staining and -visualization protocols have been described utilizing both radioactive and non-radioactive reagents, many times the use of mono-dimensional detection procedures is insufficient to address the experimental questions asked. The present review highlights the utilization of combined protein-labeling and -staining methodologies in gel electrophoresis including selected applications in polyacrylamide gels and solid membrane matrixes.
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页码:123 / 143
页数:21
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