CHARACTERIZATION OF CARE-1 - CANDIDA-ALBICANS REPETITIVE ELEMENT-1

被引：20

作者：

LASKER, BA ^{[1
]}

PAGE, LS ^{[1
]}

LOTT, TJ ^{[1
]}

KOBAYASHI, GS ^{[1
]}

MEDOFF, G ^{[1
]}

机构：

[1] WASHINGTON UNIV,SCH MED,ST LOUIS,MO 63110

来源：

GENE | 1991年 / 102卷 / 01期

关键词：

INTERSTRAIN VARIATION; SOUTHERN BLOT; DOT BLOT; GENOME ORGANIZATION; INTERSPERSED REPEAT; FIELD-INVERSION GEL ELECTROPHORESIS; PATHOGENIC YEAST;

D O I：

10.1016/0378-1119(91)90536-K

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

A middle repetitive DNA element, Candida albicans repetitive element-1 (CARE-1) has been isolated from the pathogenic yeast C. albicans. CARE-1 appears to be species-specific and constitutes approx. 0.045% of total C. albicans DNA, or a reiteration frequency of about two to twelve copies per haploid genome. The CARE-1 element has been detected on several C. albicans chromosomes separated by field-inversion gel electrophoresis, suggesting that the element is dispersed. Interstrain variation was observed in the number and distribution of hybridizing bands. The element is well conserved, since no nucleotide (nt) heterogeneity was observed when the sequences of two CARE-1 family members isolated from two different chromosomes (A and B) of C. albicans were compared. CARE-1 possesses 467 bp and is characterized by several stretches of A's and T's, short direct repeats and shows no significant homology to any known nt sequence.

引用

页码：45 / 50

页数：6

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[21] GENETICS OF CANDIDA-ALBICANS [J].