CHARACTERIZATION OF TYRAMINE AND OCTOPAMINE RECEPTORS IN THE INSECT (LOCUSTA-MIGRATORIA MIGRATORIOIDES) BRAIN

被引:55
作者
HIRIPI, L
JUHOS, S
DOWNER, RGH
机构
[1] UNIV WATERLOO,DEPT BIOL,WATERLOO N2L 3G1,ON,CANADA
[2] HUNGARIAN ACAD SCI,BALATON LIMNOL RES INST,H-8237 TIHANY,HUNGARY
基金
匈牙利科学研究基金会; 加拿大自然科学与工程研究理事会;
关键词
H-3] TYRAMINE; H-3] OCTOPAMINE; BINDING; RECEPTOR; INSECT; BRAIN;
D O I
10.1016/0006-8993(94)91530-X
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
The kinetic and pharmacological properties of [H-3]tyramine and [H-3]octopamine binding to membrane preparations of locust (Locusta migratoria migratorioides) brain were studied to characterize the tyramine and octopamine receptors. [H-3]Tyramine and [H-3]octopamine bind specifically and reversibly to the locust brain membrane with equilibrium achieved after 20 min. The dissociation of [H-3]tyramine is monophasic while that of the [H-3]octopamine shows a biphasic tendency. Scatchard analysis of the saturation curves reveals a single high affinity binding site for each of tyramine and octopamine. The mean (+/- S.E.M.) values of K-d and B-max are 6.11 +/- 0.71 nM and 21.45 +/- 3.0 fmol/mg tissue for tyramine and 5.65 +/- 0.91 nM and 15.0 +/- 2.4 fmol/mg tissue for octopamine, respectively. Pharmacological analysis of the binding suggests the presence of both tyramine and octopamine receptors in the locust brain. alpha-Adrenergic agonists and antagonists have a high affinity to the octopamine but not the tyramine receptor whereas dopaminergic drugs have a higher affinity to the tyramine receptor than the octopamine receptor. No highly effective inhibitors of tyramine binding were identified. The serotonergic blockers, mianserin, LSD, BOL are effective blockers for both tyramine and octopamine receptors, whereas the serotonergic antagonist gramine is more active against the octopamine than the serotonin receptor. The results suggest that a G-protein binding mechanism is involved in the expression of both the tyramine and octopamine effects.
引用
收藏
页码:119 / 126
页数:8
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