MEMBERS OF THE ALPHA-AMYLASE INHIBITORS FAMILY FROM WHEAT ENDOSPERM ARE MAJOR ALLERGENS ASSOCIATED WITH BAKERS ASTHMA

被引：112

作者：

GOMEZ, L

MARTIN, E

HERNANDEZ, D

SANCHEZMONGE, R

BARBER, D

DELPOZO, V

DEANDRES, B

ARMENTIA, A

LAHOZ, C

SALCEDO, G

PALOMINO, P

机构：

[1] FDN JIMENEZ DIAZ, DEPT IMMUNOL, AV REYES CATOLICOS 2, E-28040 MADRID, SPAIN

[2] ESCUELA TECH SUPER INGN AGRON, DEPT BIOQUIM, MADRID, SPAIN

[3] ALERGIA & IMMUNOL ABELLO, DEPT ANAL, MADRID, SPAIN

[4] HOSP RIO ORTEGA, SECC ALERGIA, VALLADOLID, SPAIN

来源：

FEBS LETTERS | 1990年 / 261卷 / 01期

关键词：

(wheat); Allergen; Baker's asthma; α-Amylase inhibitor;

D O I：

10.1016/0014-5793(90)80642-V

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have identified the major antigens or IgE binding components from wheat flour. Thirty-five sera from patients with baker's asthma were used to analyze the reaction with wheat salt-soluble proteins. We found a 15 kDa SDS-PAGE band which reacted with all sera tested. Purified members of the α-amylase inhibitor family, which are the main components of the 15 kDa band, were recognized by specific IgE when tested with a pool of reactive sera. Immunodetection after two-dimensional electrophoretic fractionation of crude inhibitor preparations from wheat endosperms also detected several inhibitor subunits as major low-molecular-weight allergens. © 1990.

引用

页码：85 / 88

页数：4