MOLECULAR-CLONING AND NUCLEOTIDE-SEQUENCE OF THE RHIZOBIUM-PHASEOLI RECA GENE

被引：26

作者：

MICHIELS, J ^{[1
]}

BROEK, AV ^{[1
]}

VANDERLEYDEN, J ^{[1
]}

机构：

[1] CATHOLIC UNIV LEUVEN,FA JANSSENS LAB GENET,B-3030 HEVERLEE,BELGIUM

来源：

MOLECULAR & GENERAL GENETICS | 1991年 / 228卷 / 03期

关键词：

RHIZOBIUM-PHASEOLI; RECA GENE; RECOMBINATION; DNA SEQUENCE; FEC;

D O I：

10.1007/BF00260644

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A recombinant lambda-phage carrying the recA gene of Rhizobium phaseoli was isolated from a R. phaseoli genomic library by complementation of the Fec- phenotype of the recombinant phage in Escherichia coli. When expressed in E. coli, the cloned recA gene was shown to restore resistance to both UV irradiation and the DNA alkylating agent methyl methanesulphonate (MMS). The R. phaseoli recA gene also promoted homologous recombination in E. coli. The cloned recA gene was only weakly inducible in E. coli recA strains by DNA damaging agents. The DNA sequence of the R. phaseoli recA gene was determined and compared with published recA sequences. No LexA-binding site was detected in the R. phaseoli recA upstream region.

引用

页码：486 / 490

页数：5

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