PLATELET-DERIVED GROWTH-FACTOR AND TRANSFORMING GROWTH-FACTOR-BETA-1 DIFFERENTIALLY AFFECT THE SYNTHESIS OF BIGLYCAN AND DECORIN BY MONKEY ARTERIAL SMOOTH-MUSCLE CELLS

被引:154
作者
SCHONHERR, E
JARVELAINEN, HT
KINSELLA, MG
SANDELL, LJ
WIGHT, TN
机构
[1] UNIV WASHINGTON, SCH MED SM-30, DEPT PATHOL, SEATTLE, WA 98195 USA
[2] UNIV WASHINGTON, DEPT ORTHOPED, SEATTLE, WA 98195 USA
[3] UNIV WASHINGTON, DEPT BIOCHEM, SEATTLE, WA 98195 USA
[4] VET ADM MED CTR, SEATTLE, WA 98108 USA
来源
ARTERIOSCLEROSIS AND THROMBOSIS | 1993年 / 13卷 / 07期
关键词
SMOOTH MUSCLE CELLS; PROTEOGLYCANS; GLYCOSAMINOGLYCANS; GROWTH-REGULATORY PEPTIDES;
D O I
10.1161/01.ATV.13.7.1026
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Platelet-derived growth factor (PDGF) and transforming growth factor-beta1 (TGF-beta1), two growth-regulatory peptides with opposite effects on arterial smooth muscle cell (ASMC) proliferation, were examined for their influence on the synthesis of two small chondroitin sulfate/dermatan sulfate proteoglycans (CS/DS PGs) called biglycan and decorin. Quiescent ASMCs treated with either PDGF or TGF-beta1 for 24 hours increased [S-35]sulfate incorporation into biglycan 3.3- and 2.9-fold, respectively, whereas the incorporation of [S-35]sulfate into decorin was not significantly affected. Treatment with TGF-beta1 but not PDGF more than doubled the steady-state level of messenger RNA (mRNA) transcripts hybridizing to a complementary DNA (cDNA) encoding biglycan. Both growth factors had little or no effect on steady-state levels of mRNA transcripts hybridizing to a decorin cDNA. Incorporation of [S-35]sulfate into biglycan glycosaminoglycan (GAG) was maximal by 12 to 18 hours after either PDGF or TGF-beta1 addition. Both PDGF and TGF-beta1 increased the molecular sizes of biglycan and decorin. This increase was a result of the synthesis of longer GAG chains substituted on the core proteins of both PGs. PDGF but not TGF-beta1 led to an increase of more than twofold in the ratio of 6'- to 4'-sulfated disaccharides in these newly synthesized GAG chains. These results indicate that PDGF and TGF-beta1 have specific but different effects on the synthesis of small CS/DS PGs by monkey ASMCs in culture.
引用
收藏
页码:1026 / 1036
页数:11
相关论文
共 70 条
[1]  
ASUNDI V, 1990, EUR J CELL BIOL, V52, P98
[2]   PLATELET-DERIVED GROWTH-FACTOR GENE-EXPRESSION IN HUMAN ATHEROSCLEROTIC PLAQUES AND NORMAL ARTERY WALL [J].
BARRETT, TB ;
BENDITT, EP .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (08) :2810-2814
[3]  
BASSOLS A, 1988, J BIOL CHEM, V263, P3039
[4]   EXPRESSION AND LOCALIZATION OF THE 2 SMALL PROTEOGLYCANS BIGLYCAN AND DECORIN IN DEVELOPING HUMAN SKELETAL AND NONSKELETAL TISSUES [J].
BIANCO, P ;
FISHER, LW ;
YOUNG, MF ;
TERMINE, JD ;
ROBEY, PG .
JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY, 1990, 38 (11) :1549-1563
[5]   TRANSFORMING GROWTH FACTOR-B REGULATES PRODUCTION OF PROTEOGLYCANS BY MESANGIAL CELLS [J].
BORDER, WA ;
OKUDA, S ;
LANGUINO, LR ;
RUOSLAHTI, E .
KIDNEY INTERNATIONAL, 1990, 37 (02) :689-695
[6]   NONUNIFORM INFLUENCE OF TRANSFORMING GROWTH-FACTOR-BETA ON THE BIOSYNTHESIS OF DIFFERENT FORMS OF SMALL CHONDROITIN SULFATE DERMATAN SULFATE PROTEOGLYCAN [J].
BREUER, B ;
SCHMIDT, G ;
KRESSE, H .
BIOCHEMICAL JOURNAL, 1990, 269 (02) :551-554
[7]   CHARACTERISTICS OF THE INVITRO INTERACTION OF A SMALL PROTEOGLYCAN (PG-II) OF BOVINE TENDON WITH TYPE-I COLLAGEN [J].
BROWN, DC ;
VOGEL, KG .
MATRIX, 1990, 9 (06) :468-478
[8]   EFFECT OF PLATELET-DERIVED GROWTH-FACTOR ON DNA AND PROTEIN-SYNTHESIS IN CULTURED RAT CALVARIA [J].
CANALIS, E .
METABOLISM-CLINICAL AND EXPERIMENTAL, 1981, 30 (10) :970-975
[9]  
CARLSON DM, 1968, J BIOL CHEM, V243, P616
[10]  
CHANG Y, 1983, J BIOL CHEM, V258, P5679