ASCORBIC-ACID ENHANCES FERRITIN MESSENGER-RNA TRANSLATION BY AN IRP/ACONITASE SWITCH

被引：56

作者：

TOTH, I ^{[1
]}

BRIDGES, KR ^{[1
]}

机构：

[1] HARVARD UNIV, BRIGHAM & WOMENS HOSP,SCH MED,DEPT MED, DIV HEMATOL ONCOL, BOSTON, MA 02115 USA

来源：

JOURNAL OF BIOLOGICAL CHEMISTRY | 1995年 / 270卷 / 33期

关键词：

D O I：

10.1074/jbc.270.33.19540

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Replenishment of ascorbate in cultured cells, which are almost uniformly vitamin-deficient, increases ferritin mRNA translation in response to iron by 20-fold (Toth, I., Rogers, J. T., McPhee, J. A., Elliott, S, M., Abramson, S. L., and Bridges, K. R. (1995) J. Biol, Chem. 270, 2846-2852). We now demonstrate that ascorbate increases cytosolic aconitase activity. The iron-responsive element-binding protein (IRP-1) exists in three states: bound to mRNA without aconitase activity, free in the cytosol without aconitase activity, and free in the cytosol with aconitase activity. Ascorbate converts free IRP-1 to the enzymatically active form. Enhanced ferritin synthesis with subsequent iron stimulation is due to the altered equilibrium of the free IRP-1. The cellular biology of iron is closely intertwined with that of ascorbate.

引用

页码：19540 / 19544

页数：5

共 36 条

[1] THE IRON-RESPONSIVE ELEMENT-BINDING PROTEIN - LOCALIZATION OF THE RNA-BINDING SITE TO THE ACONITASE ACTIVE-SITE CLEFT [J].

BASILION, JP ;

ROUAULT, TA ;

MASSINOPLE, CM ;

KLAUSNER, RD ;

BURGESS, WH .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1994, 91 (02) :574-578

[2]

BRIDGES KR, 1986, J BIOL CHEM, V261, P14273

[3]

BRIDGES KR, 1987, J BIOL CHEM, V262, P14773

[4] MISSING STEP IN MAN, MONKEY AND GUINEA PIG REQUIRED FOR THE BIOSYNTHESIS OF L-ASCORBIC ACID [J].

BURNS, JJ .

NATURE, 1957, 180 (4585) :552-552

[5] MODULATION OF THE RNA-BINDING ACTIVITY OF A REGULATORY PROTEIN BY IRON INVITRO - SWITCHING BETWEEN ENZYMATIC AND GENETIC FUNCTION [J].

CONSTABLE, A ;

QUICK, S ;

GRAY, NK ;

HENTZE, MW .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1992, 89 (10) :4554-4558

[6] IRON REGULATORY FACTOR EXPRESSED FROM RECOMBINANT BACULOVIRUS - CONVERSION BETWEEN THE RNA-BINDING APOPROTEIN AND FE-S CLUSTER CONTAINING ACONITASE [J].

EMERYGOODMAN, A ;

HIRLING, H ;

SCARPELLINO, L ;

HENDERSON, B ;

KUHN, LC .

NUCLEIC ACIDS RESEARCH, 1993, 21 (06) :1457-1461

[7] THE BIOCHEMICAL FUNCTIONS OF ASCORBIC-ACID [J].

ENGLARD, S ;

SEIFTER, S .

ANNUAL REVIEW OF NUTRITION, 1986, 6 :365-406

[8] ANTIOXIDANT DEFENSES AND LIPID-PEROXIDATION IN HUMAN-BLOOD PLASMA [J].

FREI, B ;

STOCKER, R ;

AMES, BN .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (24) :9748-9752

[9]

GARDNER PR, 1992, J BIOL CHEM, V267, P8757

[10] EFFECT OF GLUTATHIONE ON ACONITASE IN ESCHERICHIA-COLI [J].

GARDNER, PR ;

FRIDOVICH, I .

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1993, 301 (01) :98-102

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