FIXATION OF DNA DIRECTLY ON OPTICAL WAVE-GUIDE SURFACES FOR MOLECULAR PROBE BIOSENSOR DEVELOPMENT

被引：19

作者：

KARYMOV, MA

KRUCHININ, AA

TARANTOV, YA

BALOVA, IA

REMISOVA, LA

VLASOV, YG

机构：

[1] Department of Chemistry, St. Petersburg University, Peterhof

来源：

SENSORS AND ACTUATORS B-CHEMICAL | 1995年 / 29卷 / 1-3期

关键词：

DNA FIXATION; OPTICAL WAVE-GUIDE SENSORS; MOLECULAR PROBE BIOSENSOR;

D O I：

10.1016/0925-4005(95)01701-1

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Covalent attachment of DNA molecules to the surface of silicon nitride without an intermediate polymer layer is described. II is shown that the silicon nitride has a sorption capacity no less than 200 pg/mm(2), hybridization efficiency being close to 100%. The expected value of refractive index changes in the molecular layer under hybridization is estimated as sufficient for the operation of two kinds of waveguide-containing transducers.

引用

页码：324 / 327

页数：4

共 5 条

[1] PERFORMANCE OF A HIGHLY SENSITIVE OPTICAL WAVE-GUIDE MACH-ZEHNDER INTERFEROMETER IMMUNOSENSOR [J].